Table 3.

Optimization of LAMP assay for Salmonella detection by targeting the stn gene.

Conditions	The tested range	Difference	Good yield	Selected conditiona
Temperature	60–65°C	1°C	64–66°C	65°C
Time	0–60 min	5 min	15–60 min	25 min
Loop primers	0.5–1.0 μM	0.1 μM	0.8–1.0 μM	0.8 μM
Outer primers	0.1–0.5 μM	0.1 μM	0.2–0.5 μM	0.2 μM
MgSO4	4–8 mM	0.5 mM	6–7.5 mM	7 mM
dNTPs	0.8–2.4 mM	0.2 mM	1.2–1.8 mM	1.6 mM
Betaine	0.5–1.5 M	0.1 M	0.6–1.5 M	1.0 M
Bst DNA polymerase	4–12 units	1 unit	8–12 units	8 units

^aThe final concentrations of reagents or temperature and times used in LAMP analysis.

The concentration of FIP and BIP primers are fixed at 1.6 μM.