Table 1.
Chemokine receptor expression on the well-known CD56+low and CD56+high NK-cells observed in blood, as identified based only on the levels of CD56 and CD16 expression.
| CD56+low NK-cells | CD56+high NK-cells | P values | ||||
|---|---|---|---|---|---|---|
| CXCR1 | % (+) cells | 93.0 ± 4.5 | (85.3–99.6) | 4.0 ± 3.6 | (0.0–12.1) | <0.001 |
| MFI | 100.1 ± 19.4 | (79.3–140.9) | 7.0 ± 3.1 | (2.0–14.5) | <0.001 | |
| CV | 67.1 ± 23.6 | (51.5–145.3) | 156.8 ± 76.2 | (75.3–329.7) | <0.001 | |
|
| ||||||
| CXCR2 | % (+) cells | 91.9 ± 3.4 | (86.1–97.0) | 2.0 ± 1.4 | (0.0–4.7) | <0.001 |
| MFI | 165.7 ± 68.6 | (66.9–264.1) | 3.6 ± 1.5 | (2.2–6.8) | <0.001 | |
| CV | 64.1 ± 8.7 | (48.5–78.6) | 218.1 ± 131.4 | (82.7–568.9) | <0.001 | |
|
| ||||||
| CXCR3 | % (+) cells | 15.6 ± 11.1 | (4.8–40.1) | 97.0 ± 2.5 | (92.5–99.6) | <0.001 |
| MFI | 15.5 ± 10.7 | (6.5–41.6) | 94.5 ± 55.1 | (48.7–231.1) | <0.001 | |
| CV | 209.8 ± 62.9 | (145.1–403.4) | 62.7 ± 8.1 | (49.6–75.3) | <0.001 | |
|
| ||||||
| CXCR4 | % (+) cells | 21.8 ± 8.7 | (8.4–43.5) | 11.4 ± 4.6 | (5.5–21.4) | <0.001 |
| MFI | 11.8 ± 3.9 | (5.3–20.9) | 7.8 ± 3.1 | (3.6–14.0) | n.s. | |
| CV | 308.5 ± 89.8 | (182.6–492.8) | 318.6 ± 98.8 | (184.4–581.1) | n.s. | |
|
| ||||||
| CCR4 | % (+) cells | 0.8 ± 0.4 | (0.2–1.5) | 3.3 ± 2.9 | (0.3–9.7) | <0.05 |
| MFI | 2.2 ± 0.6 | (1.3–3.0) | 3.1 ± 1.1 | (1.5–4.4) | n.s. | |
| CV | 393.3 ± 195.1 | (138.1–677.6) | 118.7 ± 107.2 | (59.1–417.3) | 0.01 | |
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| CCR5 | % (+) cells | 13.3 ± 8.8 | (2.8–33.2) | 50.0 ± 15.3 | (24.8–78.5) | <0.001 |
| MFI | 11.1 ± 6.0 | (3.5–20.6) | 27.8 ± 18.6 | (7.7–79.9) | <0.01 | |
| CV | 353.5 ± 92.8 | (194.8–492.5) | 133.9 ± 28.1 | (96.7–192.4) | <0.001 | |
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| ||||||
| CCR6 | % (+) cells | 0.6 ± 0.4 | (0.1–1.3) | 0.9 ± 1.2 | (0.0–3.0) | n.s. |
| MFI | 2.3 ± 0.7 | (1.5–3.9) | 2.1 ± 0.8 | (1.4–4.0) | n.s. | |
| CV | 214.7 ± 126.1 | (61.4–432.8) | 106.7 ± 73.5 | (46.2–272.3) | n.s. | |
Data were obtained using the gating and analysis strategies described in Figure 1, where representative dot plots of these two conventional NK-cell subsets are presented.
Results are expressed as mean ± standard deviation (minimum–maximum) of the percentage of cells expressing each of the chemokine receptors analyzed within each CD56+ NK-cell population as well as mean ± standard deviation (minimum–maximum) of the mean fluorescence intensity (MFI) and coefficient of variation (CV) of expression.
n.s.: not statistically significant.