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. 2015 Oct 26;6:906. doi: 10.3389/fpls.2015.00906

FIGURE 1.

FIGURE 1

Colonization of plant roots by Piriformospora indica increases over time. (A) Roots of 3-day-old barley grown on 1/10 PNM agar in sterile glass jars, were inoculated with chlamydospores of P. indica. Colonization levels were determined at 3, 7, 14, and 21 dpi as the relative amount of fungal DNA by qPCR using barley (HvUBQ-60-Deg) and fungal (ITS) specific primers. Values represent the mean ± SE of two independent experiments. (B) Seven-day-old Arabidopsis seedlings were inoculated with chlamydospores of P. indica. Colonization levels were determined at 3, 7, 14, and 21 dpi as the relative amount of fungal DNA by qPCR using Arabidopsis (AtUBQ4) and fungal (ITS) specific primers. Data represents the Ct thresholds of ITS relative to the Ct thresholds of AtUBQ-4 (±SE obtain from three technical replicates of one biological experiment). Experiments were repeated twice with similar results. Asterisks indicate significance between time points at ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 analyzed by student’s t-test. (C) Analysis of PIIN_08944 expression by semi-quantitative RT-PCR. Transcripts of PIIN_08944 were detected in in vitro germinated P. indica chlamydospores (CS) grown in CM liquid medium for 7 days and in planta during colonization of Arabidopsis roots by P. indica by RT-PCR. Transcript abundance increased over time from 3 to 21 dpi. The P. indica ubiquitin (UBQ) gene served as reference.