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. 2015 Oct 26;6:906. doi: 10.3389/fpls.2015.00906

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Copyright © 2015 Akum, Steinbrenner, Biedenkopf, Imani and Kogel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assessment of flg22 and chitin-induced reactive oxygen species (ROS) accumulation in barley and Arabidopsis tissue. Leaf disks of 3-week-old barley HvPIIN_08944-OE or EV control plants were treated with (A) 100 nM flg22, and (B) 200 mg/ml crab shell chitin. (C) Leaf disks of 4-week-old AtPIIN_08944-OE or wt Arabidopsis plants were treated with 100 nM flg22. ROS was determined by measuring the relative light unit over time with a luminol-chemiluminescence assay using a Tecan reader. Error bars represent the mean ± SE of three independent experiments. (RLU, relative light units; EV, empty vector).