Table 2.
Summary of predominant long-fragment bacterial 16S rRNA gene sequences (nt > 1200 bp) obtained from thermal vent biomass samples from Yellowstone Lake using clone-library analysis (n ~ 48 per site).
| Location year (ID) | Closest cultivated relativea | Taxonomic groupb | Percent library | Clone ID | IDY (%) | Relative NCBI No. |
|---|---|---|---|---|---|---|
| Inflated Plain | Sulfurihydrogenibiumsp. Y04ACS1 | Aquificales | 83 | 329-10-20 | ~99 | AM259493.1 |
| Geothermobacterium ferrireducens | Thermodesulfobacteria | 5 | 329-5 | 98.6 | AF411013.1 | |
| 2007 (329S) | Caldisericum exile AZM16c01 | Candid. Div. OP5 | 5 | 329-21 | 98.3 | NR_075015.1 |
| Thiovirga sulfuroxydans | Chromatiales | 5 | 329-22 | 97.3 | NR_040986.1 | |
| 2008 (348S) | Sulfurihydrogenibium sp. Y04ACS1 | Aquificales | 100 | 348-5 | >99 | AM259493.1 |
| 2008 (359S) | Sulfurihydrogenibium sp. Y04ACS1 | Aquificales | 100 | 359-5 | >99 | AM259493.1 |
| West Thumb | Sulfurihydrogenibium yellowstonense | Aquificales | 30 | 339-15,16 | >99 | JQ346738.1 |
| Thiovirga sulfuroxydans | Chromatiales | 20 | 339-20, 24 | 97.3 | NR_040986.1 | |
| 2007 (339) | Methylothermus thermalis MYH | Methylococcales | 10 | 339-13 | 92.8 | NR_043209.1 |
| Caldisericum exile AZM16c01 | Candid. Div. OP5 | 10 | 339-17 | 98.5 | NR_075015.1 | |
| 2007 (342) | Methylothermus thermalis MYH | Methylococcales | 64 | 342-4 | 93.0 | NR_043209.1 |
| Sulfurihydrogenibium sp. Y03AOP1 | Aquificales | 10 | 342-12 | 99.4 | NR_074557.1 | |
| Thiobacillus denitrificans | Hydrogenophilales | 10 | 342-5 | 91.0 | NR_074417.1 | |
| Curvibacter sp. PL21 | Burkholderiales | 3 | 342-7 | 98.9 | KF206393.1 | |
| Thermus aquaticus | Thermales | 3 | 342-9 | 95.4 | NR_025900.1 | |
| Bellilinea sp. clone 96 | Nov. Chloroflexi | 3 | 342-8 | < 95 | JQ183076.1 | |
| 2008 (369S) | Methylothermus thermalis MYH | Methylococcales | 28 | 369-20 | 92.8 | NR_043209.1 |
| Sulfurihydrogenibium sp. Y04ACS1 | Aquificales | 25 | 369-21 | 99.6 | AM259493.1 | |
| Thiobacillus dentrificans | Hydrogenophilales | 6 | 369-25 | 96.1 | NR_074417.1 | |
| Geothermobacterium ferrireducens | Thermodesulfobacteria | 3 | 369-19 | 98.3 | AF411013.1 | |
| Fervidobacterium sp. CBS-3 | Thermotogales | 3 | 369-18 | 91.3 | EF222230.1 | |
| Syntrophomonas palmitatica MPA | Syntrophobacterales | 3 | 369-22 | 94.5 | NR_041528.1 | |
| Thermodesulforhabdus norvegica | Syntrophobacterales | 3 | 369-23 | 91.8 | NR_025970.1 | |
| Rhodoferax ferrireducens | Burkholderiales | 3 | 369-27 | 97.5 | NR_074760.1 | |
| Thermus aquaticus | Thermales | 3 | 369-24 | 98.0 | NR_025900.1 | |
| Desulfomicrobium thermophilum P6.2 | Uncl. Proteobacteria | 3 | 369-16 | 96.8 | NR_042924.1 | |
| Pelosinus sp. UFO1 | Firmicutes | 3 | 369-268 | 94.0 | DQ295866.1 | |
| Mary Bay | Caldisericum exile AZM16c01 | Candid. Div. OP5 | 12 | 349-20 | 89.9 | NR_075015.1 |
| Thermanaerothrix daxensis GNS-1 | Chloroflexi | 12 | 349-10 | 92.9 | HM596746.1 | |
| 2008 (349S) | Cystobacter violaceus Cbvi34 | Myxococcales | 12 | 349-11 | 93.2 | KF267724.1 |
| Desulfosarcina cetonica | Desulfurobacteriales | 12 | 349-12 | 92.1 | NR_028896.1 | |
| Ornatilinea apprima | Chloroflexi | 8 | 349-15 | 88.9 | NR_109544.1 | |
| Prosthecobacter fluviatilis | Verrucomicrobiales | 8 | 349-21 | 83.5 | NR_041608.1 | |
| Methylobacter psychrophilus Z-0021 | Methylococcales | 8 | 349-17 | 98.5 | NR_025016.1 | |
| Geobacter daltonii | Desulfomonadales | 4 | 349-13 | 84.4 | NR_074916.1 | |
| Syntrophus aciditrophicus SB | Syntrophobacterales | 4 | 349-19 | 83.2 | NR_102776.1 | |
| Aquihabitans daechungensis | Acidimicrobiales | 4 | 349-14 | 93.5 | NR_132289.1 | |
| Elliot's Crater | Thiobacillus thioparus DSM505 | Hydrogenophilales | 57 | 351-8 | 96.8 | NR_117864.1 |
| Bellilinea caldifistulae | Chloroflexi | 14 | 351-1 | 90.4 | NR_041354.1 | |
| 2008 (351) | Sulfurihydrogenibium sp. Y04ACS1 | Aquificales | 9 | 351-5 | 98.9 | AM259493.1 |
| Pelobacter massiliensis DSM6233TN | Desulfuromonadales | 4 | 351-4 | 85.3 | FR749901.1 | |
| Denitratisoma sp. TSA61 | Rhodocyclales | 4 | 351-132 | 94.9 | AB542411.1 | |
| Thermomonas hydrothermalis SGM-6 | Xanthomonadales | 2 | 351-7 | 98.0 | NR 025265.1 | |
| Caldisericum exile AZM16c01 | Candid. Division OP5 | 2 | 351-130 | 97.4 | NR 075015.1 | |
| Otter Vent | Fervidobacterium changbaicum | Thermotogales | 27 | 332-7 | 94.7 | NR 043248.1 |
| Synechococcus sp. TS-97 B' | Cyanobacteria | 11 | 332-16 | 99.7 | AY884056.1 | |
| 2007 (332) | Anaeromyxobacter sp. K | Myxococcales | 11 | 332-13 | 86.0 | NR 074969.1 |
| Thermodesulfoba. hydrogeniphilum | Thermodesulfobacteria | 11 | 332-17 | 79.6 | NR 025146.1 | |
| Chloroflexus sp. strain 396-1 | Chloroflexi | 8 | 332-5 | 98.1 | AJ308498.1 | |
| Thiobacter subterraneus C55 | Burkholderiales | 8 | 332-18 | 92.1 | NR 024834.1 | |
| Acidobacteria bacterium KBS 96 | Acidobacteriales | 5 | 332-3 | 91.8 | FJ870384.1 | |
| Fischerella sp. JSC-11 | Cyanobacteria | 5 | 332-12 | 99.9 | HM636645.1 | |
| Anaerolinea thermophila UNI-1 | Anaerolineales | 3 | 332-4 | 98.6 | AP012029.1 |