. 2015 Oct 26;5:15570. doi: 10.1038/srep15570

Table 1. The concentration of free and protein-associated BMAA in neonatal rat tissues 24 hours after exposure to BMAA on PND 9-10.

Tissue	Dose (mg/kg)	Free BMAA (ng/mg wet tissue)	SEM	Protein-associated BMAA (ng/mg wet tissue)	SEM	Fraction protein-associated BMAA of total BMAA (%)¹
Hippocampus	2 × 40	1.964	0.396	0.011	0.002	0.6
	2 × 150	39.809***	5.534	0.177**	0.034	0.4
	5 × 40	6.512*	1.164	0.044*	0.005	0.7
Striatum	2 × 40	4.065	0.726	ND^##	–	ND^##
	2 × 150	47.077***^{, #}	5.239	0.048**^{, #}	0.012	0.1**^{, ##}
	5 × 40	9.510**	0.711	0.024**^{, #}	0.002	0.2**^{, #}
Cortex	2 × 40	4.551^#	0.723	ND^##	–	ND^##
	2 × 150	61.839***^{, #}	4.359	0.081**	0.022	0.1**^{, ##}
	5 × 40	7.640	1.531	0.015**^{, #}	0.006	0.2**^{, #}
Hypothalamus	2 × 40	9.170	2.657	0.021^##	0.001	0.2
	2 × 150	81.251*^{, #}	20.809	0.267**	0.045	0.3
	5 × 40	14.992	5.190	0.043*	0.009	0.3
Cerebellum	2 × 40	2.692	0.336	ND^##	–	ND^##
	2 × 150	37.270***	3.152	0.040**^{, #}	0.010	0.1**^{, ##}
	5 × 40	4.347	0.753	0.010**^{, ##}	0.002	0.2**^{, #}
Liver	2 × 40	47.63^###	6.66	1.23^###	0.13	2.52^###
	2 × 150	174.44***^{, ###}	9.10	6.45***^{, ###}	0.42	3.56*^{, ###}
	5 × 40	66.77*^{, ###}	3.77	1.03^###	0.10	1.51*^{, #}
Pancreas	2 × 40	1.143	0.182	0.011	0.001	0.94
	2 × 150	22.353***^{, #}	2.796	0.137**	0.016	0.61*
	5 × 40	2.525**^{, #}	0.180	0.021*^{, ##}	0.003	0.81
Skeletal muscle	2 × 40	11.494^###	0.984	0.033^###	0.003	0.28^#
	2 × 150	75.358***^{, ##}	6.210	0.187**	0.035	0.25^#
	5 × 40	7.867*	0.939	0.027^#	0.002	0.34
Spleen	2 × 40	1.423	0.097	0.010	0.003	0.61
	2 × 150	11.564***^{, ##}	0.121	0.075*^{, #}	0.021	0.65
	5 × 40	2.060**^{, ##}	0.058	0.028	0.009	1.34
Thymus	2 × 40	0.819^#	0.125	ND^##	–	ND^##
	2 × 150	13.207***^{, ##}	1.668	0.076**^{, #}	0.006	0.6**
	5 × 40	1.435*^{, ##}	0.117	0.012**^{, ##}	0.001	0.8**

ND: not detectable, i.e. below 0.01 ng/mg wet tissue. Values represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the group administrated 2 × 40 mg/kg BMAA, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared with hippocampus in respective treatment group (ANOVA followed by Student's t-test test. Chi-square test was used when groups had not detectable levels of protein bound BMAA; n = 4).

¹Defined as concentration protein-associated BMAA/(free BMAA + protein-associated BMAA)