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. 2015 Oct 26;5:15545. doi: 10.1038/srep15545

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Temperature functions of ΔH and ΔS were obtained using equations (2) and (3), respectively, in which each temperature function of ΔCp was used for the calculation of Ec0VV and Ec0VV_6. The green, red, and blue curves represent values of ΔG(=ΔH–TΔS), ΔH, and TΔS, respectively. Numbers 1 and 2 represent Ec0VV_6 and Ec0VV, respectively. Open circles with error bars show the ΔH value of each protein at the denaturation temperature, as indicated in the figure.