Table 1. Denaturation enthalpy of examined EcCutA1_0SH mutants at denaturation temperatures.

	Td( °C)*			ΔH (kJ mol−1)*
EcCutA1_0SH	85.6	±	0.3	870	±	21
EcCutA1_0SH_S11V	102.7	±	0.3	1302	±	21
EcCutA1_0SH_E61V	101.0	±	0.2	1228	±	15
Ec0VV**	113.2	±	0.2	1569	±	15
Ec0VV_T17K	107.2	±	0.3	1315	±	15
Ec0VV_S48D	105.4	±	0.2	1242	±	14
Ec0VV_T17K/S48D	112.0	±	0.1	1387	±	22
Ec0VV_A39D	105.4	±	0.5	1197	±	31
Ec0VV_S48 K	112.2	±	0.9	1173	±	35
Ec0VV_A39D/S48 K	118.3	±	0.7	1410	±	33
Ec0VV_H72 K	118.4	±	0.4	1521	±	34
Ec0VV_S82 K	116.9	±	0.5	1479	±	22
Ec0VV_S82R	117.1	±	0.5	1446	±	24
Ec0VV_T88R	117.6	±	0.6	1501	±	58
Ec0VV_Q87 K	116.8	±	0.5	1424	±	13
Ec0VV_Q87K/T88R	122.4	±	0.6	1582	±	56
Ec0VV_S110R	117.3	±	0.4	1637	±	35
Ec0VV_6***	136.8	±	0.9	1739	±	59

^*Average value and its standard deviation of at least 6 data.

^**Ec0VV represents EcCutA1_0SH_S11V/E61V mutant.

^***Ec0VV_6 represents Ec0VV_A39D/S48K/H72 K/S82 K/Q87 K/T88R mutant.