Table 2. Thermodynamic parameters of denaturation for EcCutA1_0SH mutants at the denaturation temperature (113.2 ^oC) of Ec0VV.

	ΔH		TΔS		ΔG (=ΔΔG)		ΔΔH		TΔΔS
	a*	b*	a	b	a	b	a	b	a	b
EcCutA1_0SH	1175	1254	1255	1336	−80	−82	−394	−315	−314	−233
EcCutA1_0SH_S11V	1396	1440	1434	1478	−38	−38	−173	−129	−135	−91
EcCutA1_0SH_E61V	1340	1377	1382	1419	−42	−42	−229	−192	−187	−150
Ec0VV	1569	1569	1569	1569	0	0	0	0	0	0
Ec0VV_A39D	1265	1289	1290	1315	−26	−26	−304	−280	−279	−254
Ec0VV_S48 K	1181	1184	1184	1187	−3	−3	−388	−385	−385	−382
Ec0VV_A39D/S48 K	1375	1358	1356	1340	18	18	−194	−211	−213	−229
Ec0VV_H72 K	1485	1469	1465	1449	20	20	−84	−100	−104	−120
Ec0VV_S82 K	1453	1442	1439	1428	14	14	−116	−127	−130	−141
Ec0VV_T88R	1470	1456	1453	1440	17	17	−99	−113	−116	−129
Ec0VV_Q87 K	1398	1387	1385	1374	13	13	−171	−182	−184	−195
Ec0VV_Q87 K/T88R	1524	1495	1488	1459	36	36	−45	−74	−81	−110
Ec0VV_S110R	1608	1595	1590	1578	17	17	39	26	21	9
Ec0VV_6	1638	1560	1540	1465	98	96	69	−9	−29	−104

The unit is kJ mol⁻¹.

^*a and b represent the calculated results using the temperature function of ΔCp obtained from Ec0VV and Ec0VV_6, respectively.