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. 2015 Oct 26;5:15583. doi: 10.1038/srep15583

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Copyright © 2015, Macmillan Publishers Limited

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Nucleosome matching percentage is the percentage value of the number of matched nucleosomes between two replications (Rep1 and Rep2) relative to the total number of identified nucleosomes in the first replication (Rep1) under a certain deviation. The deviation varied from 1 bp to 60 bp. The dyad positions of nucleosomes were identified with a wavelet transformation-based peak finding algorithm. Nucleosome matching percentage between two biologic replications was calculated for H3S10A, H3K56A, H4K20A, and H4K16A mutant strains and for H3 and H4 wild-type (WT) strains, respectively. The matching percentage between the WT and mutant H3K56A is also shown. The green line shows the average matching percentage between the WT (WT H2A-H2B versus WT H3-H4) in the first experiment (Rep1). The blue line indicates the matching percentage between two replications of WT H3-H4.