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. 2015 Oct 26;5:15584. doi: 10.1038/srep15584

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Copyright © 2015, Macmillan Publishers Limited

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(a) Syncytium induced through aMPV F proteins at low or neutral pH (magnification: 100×, 8 cm ×8 cm as shown). (b,c) The fusogenic activity of aMPV F proteins exposed to low or neutral pH was analyzed through syncytium formation and reporter gene assays. P < 0.05 and P < 0.005 are respectively presented as **and ***. (d) The time interval between transfection and low pH treatment influences the fusogenic activity of aMPV/C F protein. aMPV/C F protein was treated with low pH at 0, 12, or 24 h post-transfection. At 48 h post-transfection, the fusogenicity of aMPV/C F protein was analyzed. The data are presented as the ratio of the aMPV/C F protein at low pH vs. aMPV/C F protein at neutral pH.