Figure 4. Ablation of β-arr1 Reduces NSC Activation and Neuronal Production in Adult DG.

(A,B) Sample projected confocal images and stereological quantification of Nestin⁺ RGLs in DG of P21, 3 and 6 month-old mice. The number of RGLs was normalized to the GCL volume; two-way ANOVA, post hoc Tukey test. (C,D) Sample projected confocal images and stereological quantification of Nestin⁺ GFAP⁺ RGLs and Nestin⁺ GFAP⁺ MCM2⁺ RGLs in the DG of WT and KO mice. Orthogonal images ((C), bottom) are shown to confirm colocalization. (E,F) Confocal images and quantification of Tbr2⁺ IPCs and Tbr2⁺ MCM2⁺ IPCs in SGZ of WT and KO mice. (G) Schematic of experimental schedule. WT and β-arr1 KO mice were injected with BrdU (i.p., 100 mg/kg) for 7 days, and sacrificed 30 days after the last BrdU injection. (H,I) Sample projected confocal images and stereological quantification of BrdU⁺ NeuN⁺ cells in DG. High magnification orthogonal images show the colocalization of BrdU and NeuN. Data are mean ± s.e.m.; n = 5–6 per genotype; *p < 0.05 versus WT, t-test; Scale bar, 50 μm.