Table 1. qPCR validation of three differentially expressed miRs in microarray data.
| miR-133b |
miR-206 |
miR-302b |
||||
|---|---|---|---|---|---|---|
| miR array | qPCR | miR array | qPCR | miR array | qPCR | |
| L33 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| L65 | 2.2 | 9.3 | 2.1 | 20.0 | 0.8 | 0.8 |
| L90 | 5.7 | 16.8 | 1.9 | 27.7 | 0.7 | 0.6 |
| T33 | 2.3 | 2.2 | 1.2 | 3.9 | 1.0 | 1.0 |
| T65 | 3.5 | 10.1 | 1.9 | 35.4 | 0.6 | 0.7 |
| T90 | 7.3 | 15.7 | 4.0 | 69.7 | 0.5 | 0.5 |
| W33 | 21.1 | 159.7 | 1.8 | 15.3 | 0.4 | 0.5 |
| W65 | 14.1 | 18.7 | 5.5 | 44.0 | 0.4 | 0.5 |
| W90 | 5.1 | 15.3 | 3.9 | 35.9 | 0.8 | 0.8 |
Note: The qPCR row provides the ratio of the 2−ΔΔCt of L3346. For the L33 sample, the fold change in gene expression relative to the L33 equals one, by definition. miR array row provides the expression abundance of each breeds/stage sample relative to L33 in the microarray data. L, Landrace; T, Tongcheng; W, Wuzhishan; 33, 65 and 90 refer to days post coitus.