Table 2. qPCR validation of 12 differentially expressed genes in oligoarray data.

Gene	Method	L33	L65	L90	T33	T65	T90	W33	W65	W90
COL15A1	Pig oligoarray	1.00	2.29	5.89	1.39	2.16	5.64	0.54	3.51	6.24
	qPCR	1.00	1.96	1.95	0.88	1.51	1.56	0.41	1.00	1.78
TPM3	Pig oligoarray	1.00	0.27	0.44	1.31	3.14	2.78	0.06	0.36	0.50
	qPCR	1.00	0.34	0.24	1.07	3.42	2.16	0.00	0.68	0.92
IGF2	Pig oligoarray	1.00	2.14	4.45	1.13	2.20	2.11	0.19	2.23	3.55
	qPCR	1.00	1.80	2.38	1.11	2.13	2.89	0.00	3.90	4.43
CSRP3	Pig oligoarray	1.00	3.04	4.41	1.26	2.79	3.93	0.99	3.31	3.83
	qPCR	1.00	1.38	1.48	0.64	0.71	0.98	0.96	1.41	1.89
LAMA2	Pig oligoarray	1.00	3.41	3.84	1.21	2.27	3.41	0.25	2.88	3.77
	qPCR	1.00	1.21	1.56	0.58	0.76	1.15	0.75	0.87	0.82
LAMA3	Pig oligoarray	1.00	5.54	10.71	0.52	4.63	13.22	0.64	5.79	9.12
	qPCR	1.00	18.74	23.41	1.16	15.61	66.07	1.84	11.88	15.74
LAMB2	Pig oligoarray	1.00	1.82	3.62	0.56	1.03	1.81	0.10	0.60	2.08
	qPCR	1.00	1.55	1.74	1.92	2.03	2.55	1.19	1.55	1.68
UCHL1	Pig oligoarray	1.00	0.09	0.06	0.96	0.13	0.11	0.42	0.10	0.07
	qPCR	1.00	0.15	0.03	1.32	0.06	0.05	2.06	0.05	0.03
FNDC1	Pig oligoarray	1.00	1.86	0.98	0.72	1.90	0.75	0.21	1.23	1.07
	qPCR	1.00	2.15	0.60	0.44	1.33	0.48	0.38	0.64	0.32
FEZ2	Pig oligoarray	1.00	3.04	5.01	0.46	2.96	3.67	0.66	4.34	5.04
	qPCR	1.00	3.17	3.90	1.62	2.53	3.09	1.88	3.49	4.31
FGL2	Pig oligoarray	1.00	15.85	19.52	2.40	6.34	26.36	6.18	32.29	39.04
	qPCR	1.00	16.46	20.36	1.72	17.33	42.52	1.09	21.93	37.07
IGFBP7	Pig oligoarray	1.00	2.12	4.61	1.24	2.04	3.69	0.37	1.52	3.47
	qPCR	1.00	1.20	1.28	1.1	1.06	2.20	1.14	1.20	2.38

Note: The qPCR row provides the ratio of the 2^−ΔΔCt of L33⁴⁶. For the L33 sample, the fold change in gene expression relative to the L33 equals one, by definition. The microarray row provides the expression abundance of each breeds/stage sample relative to L33 in the oligoarray data. L, Landrace; T, Tongcheng; W, Wuzhishan; 33, 65 and 90 refer to days post coitus.