Table 5. Primer sequences of miRNAs and genes selected for validation by qPCR.

Gene	Accession ID	Primer sequence	TM (°C)	Product size (bp)
miR-206	MI0013084	RT: CTCAACTGGTGTCGTGGAGTCGGCA
		ATTCAGTTGAGTCACACA
miR-133b	MI0013089	RT: CTCAACTGGTGTCGTGGAGTCGGCA
		ATTCAGTTGAGTCGCTGGT
miR-302b	MI0000772	RT: CTCAACTGGTGTCGTGGAGTCGGCAA
		TTCAGTTGAGCTACTA
miR-206	MI0013084	Forward 5′-GGGTGGAATGTAAGGAAG-3′	60	62
		Reverse 5′-CTCAACTGGTGTCGTGGAGTC-3′
miR-133b	MI0013089	Forward 5′-GGGTTTGGTCCCCTTCA-3′	60	62
		Reverse 5′-CTCAACTGGTGTCGTGGAGTC-3′
miR-302b	MI0000772	Forward 5′-GGGTAAGTGCTTCCATGTTT-3′	60	62
		Reverse 5′-CTCAACTGGTGTCGTGGAGTC-3′
U6	EU520423	Forward 5′-GCTTCGGCAGCACATATACTAAAAT-3′	60	84
		Forward 5′-CGCTTCACGAATTTGCGTGTCAT-3′
GAPDH	NM_001206359.1	Forward 5′-GGGCATGAACCATGAGAAGT-3′	60	233
		Reverse 5′-AAGCAGGGATGATGTTCTGG-3′
IGF2	EST contig	Forward 5′-5′CACCATCCACCTCGTGACCT-3′	59	92
		Reverse 5′-GAGATGCCCGCAGACAGAA-3′
TPM3	EST contig	Forward 5′-GGAACTCCAGGAAATCCAACTC-3′	59	214
		Reverse 5′-TTCTTCAGCAGCACTCAGACACT-3′
COL15A1	EST contig	Forward 5′-CAGAAACCTGGTGACAGCATT-3′	60	236
		Reverse 5′-TTGAAATGGATGTCAGCGGAA-3′
CSRP3	NM_001172368.1	Forward 5′-GATCGGCTATGGACAAGGTGC-3′	58	249
		Reverse 5′-CTCTTCCCACAGATGGCACAG-3′
LAMA2	XM_001926517.2	Forward 5′-GCCCTGATTATGTGGGAGTTA-3′	60	220
		Reverse 5′-GCCTGTCCAGTCTGCCTTCGT-3′
LAMA3	XM_003127861.1	Forward 5′-ACTTTGGAAGCACCTACTCAC-3′	60	186
		Reverse 5′-GACGACCATTTATCAAGGACAC-3′
LAMB2	EST contig	Forward 5′-GAGGCAATGGTTGACACACA-3′	60	158
		Reverse 5′-AGGCTATTCCCTGCTCGTTT-3′
UCHL1	NM_213763.2	Forward 5′-CAGTAGCCAATAATCAGGACA-3′	56	243
		Reverse 5′-TCCGACCATCAAGTTCATAGAG-3′
FNDC1	EST contig	Forward 5′-ATCTGGCTGGAAAGAAACGCT-3′	60	230
		Reverse 5′-GTCCCAGTCCACAATGACGAAT-3′
FEZ2	EST contig	Forward 5′-CAGGACCTTGCATTTGCTTAC-3′	62	235
		Reverse 5′-ATCTGACTGGGTGGGCGTTTC-3′
FGL2	NM_001005152.2	Forward 5′-CCAACAATGAGACGGAGGA-3′	58	261
		Reverse 5′-CTGGGTCTCGGTTGTCGT-3′
IGFBP7	NM_001163801.1	Forward 5′-CCATCGTGACACCCCCTAAG-3′	60	292
		Reverse 5′-GAAGCCTGTCCTTGGGAGTTA-3′