Table 6. Information on primers corresponding to target genes and miRNA mimics.
Primer symbol | Primer sequence (5′-3′) | Size (bp) | TM (°C) |
---|---|---|---|
CNN3-CDS(V)-F | CTAGCTAGCATGACCCACTTCAACAAGGG | NheI | |
CNN3-CDS(V)-R | CCGCTCGAGCTAATAATCAATGCCCTGGTCG | XhoI | |
CNN3-3′UTR-F | GTTCACGGGGGAGCTCA | 689 | 60.0 |
CNN3-3′UTR-R | GATACATTGGCACAAACAG | ||
RAP2C-3′UTR-F | AGATTGTAAGGGTGGAGGCA | 532 | 57.0 |
RAP2C-3′UTR-R | AACAACTCCTAAACAGATGCCA | ||
MAP1A-3′UTR-F | CCCTCCGTATCTGAATGTCT | 634 | 55.0 |
MAP1A-3′UTR-R | TCTTAGTCGGGCGGTAGTCT | ||
SFRS3-3′UTR-F | TTGTAGTTGAGCAAGCAGTC | 721 | 54.0 |
SFRS3-3′UTR-R | ATGGACTTTTTGAACTGGCT | ||
SMARCD1-3′UTR-F | CCTGCCTTGGTCTTGCTT | 1574 | 55.0 |
SMARCD1-3′UTR-R | ATTAAAACTGGGTGACATCG | ||
miR-133b | UUUGGUCCCCUUCAACCAGCUAU | ||
miR-206 | UGGAAUGUAAGGAAGUGUGUGA |
Note: F, forward primer; R, reversed primers.