FIG 4 .

PLC and IP₃R are required for Ca²⁺ release from ER. (A) ED cells were treated with U73122 or (−)-xestospongin C (Xesto) for 30 to 60 min at 37°C prior to infection. The cells were washed, loaded with Fura-2AM, and exposed to EHV-1. Release of Ca²⁺ was monitored, and images were taken at the indicated time points. Viruses were added at the 50-s time point. (B) Fluorescence intensity of Fura-2AM as a function of time after exposure of ED cells to EHV-1 in the presence or absence of U73122 or (−)-xestospongin C. The lines show averages from 3 independent experiments. P < 0.001 indicates a significant difference between EHV-1 in the presence and absence of the used inhibitors.