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. 2015 Oct 26;5:15282. doi: 10.1038/srep15282

Figure 2. HSM alleviates BLM–induced lung fibrosis and inflammatory cells accumulation in mice.

Figure 2

(a) Experimental design: Mice were instilled intratracheally with BLM (1.5 mg/kg in 50 μl of PBS). The treatment group received HSM ethanol extract (10%) orally for a week before BLM instillation. The mice were sacrificed on day 7 and 21 following BLM instillation, and lung samples were collected for further analysis. (b) Paraffin sections from lung tissues of the mice on day 21 were stained with H&E or Masson’s trichrome. Scale bars = 50 μm. (c) Quantitative examination of the effects of HSM ethanol extract on BLM–induced lung fibrosis. (d–g) BALF was collected 7 days after BLM treatment to quantify total cells, macrophages, neutrophils, and lymphocytes. The values shown represent means ± SEM (n = 5 in each group). #P < 0.05 versus PBS + EtOH group. *P < 0.05 versus BLM + EtOH group.