Figure 5.

IFN-α2b treatment downregulates SDF-1/CXCR4 signal in burn patients. (A) Immunohistochemistry staining for SDF-1 in HTS tissue from burn patients before and after IFN-α2b treatment. (B) Under a light microscope, a 500×1500 pixel field was defined through the whole layers of sections, and the signal was quantified by MetaMorph analysis. The data are displayed as a percentage of target staining in tissue. (C) Representative flow cytometry dot plots of CD14⁺CXCR4⁺ cells in PBMCs from a burn patient before and after IFN-α2b treatment. (D) Regression analysis showing the changes individually of CD14⁺hiCXCR4⁺ cell populations of total PBMCs from burn patients treated with or without IFN-α2b treatment. Data represent mean±SE (n=3). To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/wound