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. 2015 Jul 1;24(21):2536–2546. doi: 10.1089/scd.2015.0018

FIG. 4.

FIG. 4.

Substituting B27 for other supplements in Protocol 4. (A) Formulation comparison of supplements. (B) The percentage of eGFP+ cells after 4-day DE differentiation of S17d5 cells. (C) Total live cell numbers at the end of 4-day DE differentiation of S17d5 cells. (D) Total live eGFP+ cell numbers at the end of 4-day DE differentiation of S17d5 cells. Independent runs were normalized to 1.0×106 starting undifferentiated cells (dotted line). *P<0.05 compared with the Mod-D'Amour protocol by Student's unpaired t-test. Data are presented as mean±SEM, n=3–4.