FIG. 4.

(A) Representative proteome profiler arrays, showing the presence of pro- (marked in green) and antiangiogenic (marked in red) factors in secretomes collected under (a) SDM (N), (b) SDM (H+G), (c) SDM (H−G), and (d) negative control (plain, nonconditioned SDM containing 2% FBS). Graphics (e–h) show the relative expression levels of angiogenic factors in each secretome as well as of the common secreted factors in all three different secretomes. Values were normalized to the positive reference dots of each membrane and are expressed as means ± standard deviations (asterisks indicate statistically significant differences of secreted factors under H+G and H−G conditions compared with N, *P < 0.05, **P < 0.01). (B) Enzyme-linked immunosorbent assays (ELISA) for quantification of (a) vascular endothelial growth factor (VEGF), (b) transforming growth factor beta 1 (TGF-β1), and (c) fibroblast growth factor-2 (FGF-2) concentrations (pg/mL) in SCAP secretomes collected under different stress conditions (groups 1–6). Baseline VEGF, TGF-β1, and FGF-2 values in respective nonconditioned media (CCM and SDM) were subtracted from values obtained in the assay to calculate the final growth factor concentrations in each secretome. Results are expressed as means ± standard deviations of three independent experiments in duplicates of secretomes collected from three different cell donors [black asterisks indicate statistically significant differences between N, H+G, and H−G conditions within the same serum concentration group (CCM or SDM), and gray asterisks (in the rectangular frame) indicate statistically significant differences between CCM and SDM for similar oxygen/glucose conditions, that is, CCM (N) vs. SDM (N), CCM (H+G) vs. SDM (H+G), and CCM (H−G) vs. SDM (H−G), *P < 0.05, **P < 0.01]. Color images available online at www.liebertpub.com/scd