FIG. 6.

Representative quantification data and light microscope microphotographs (scale bars 100 μm) of the in vitro capillary-like network formation assay and respective blocking experiments, showing the number of master junctions, number of master segments, and total master segment length after exposure of HUVECs to SCAP secretomes collected under different stress conditions (groups 1–6). Results are expressed as means ± standard deviations of three independent experiments in duplicates [black asterisks indicate statistically significant differences between N, H+G, and H−G conditions within the same serum concentration group, that is, CCM or SDM, and gray asterisks (in the rectangular frame) indicate statistically significant differences of each of the blocked (with anti-VEGF and anti-TGF-β1) secretomes compared with the total secretome within the same group, *P < 0.05, **P < 0.01]. Positive control: CCM (containing 15% FBS) and negative control: SDM (containing 2% FBS). Color images available online at www.liebertpub.com/scd