FIG. 4.

Effect of Rho GTPase on cell cytoskeleton dynamics. Cells were transfected with Rac1 and Cdc42 siRNA (20 nM) or Nt siRNA for 1 day before treatment of 8-Bromo cAMP for 24 h and expression of PAK, Arp3, N-WASP, TOCA, and p-cofilin was detected by western blot (A) and immunofluorescence staining (B). The right panel of (A) depicted by bars denotes mean ± S.E. of three experiments for each condition determined by densitometry. *P <0.05 versus control. **P < 0.05 versus 8-Bromo cAMP alone. (C) Cells were transfected with Arp, PAK, TOCA, N-WASP siRNA (20 nM), or Nt siRNA for 1 day before treatment of 8-Bromo cAMP for 4 h. The actin cytoskeleton dynamics was detected by immunofluorescence staining. The example shown is representative of three independent experiments. Color images available online at www.liebertpub.com/scd