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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Evol Dev. 2015 Nov;17(6):367–379. doi: 10.1111/ede.12167

Figure 5. Co-localization of a canonical Wnt reporter (TCFsiam:mCherry) with EdU-488 mitotic labeling using in vitro explant cultured maxillary barbels/.

Figure 5

A) Two-channel rendering of a developing zebrafish maxillary barbel; the distal end, which is digitally truncated, points right. mCherry+ cells (red) occupy a cylindrical, mesenchymal sheath surrounding the barbel core. Numerous EdU+ cells (green) are superficial to this layer, indicating maximal cell division in the overlying epithelium. Only two ventral cells are double-labeled (mCherry+/EdU+).

B) mCherry+ cells from a second double-labeled specimen.

C) EdU+ cells (green) from the same region as B.

D) Merged image of B and C. The two yellow nuclei (*) are double-labeled.