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. 2015 Oct 26;15:236. doi: 10.1186/s12866-015-0551-0

Table 2.

Genes and primers used for Multilocus sequence typing

Gene Amplicon size (bp) Application size (bp) Gene function PCR primer Sequences (5’-3’) Temperature
carB 652 499 carBamoyl phosphate synthetase carB_primerF AAGGCTACAGTGTTGTTCT 51 °C
carB_primerR GATAAGGTTTGCGTTGG
clpX 768 630 ATP-dependent Clp protease subunit X clpX_primerF GCAGGTTATGTGGGTGA 52 °C
clpX_primerR TACTGGAGCAGCTTTCC
dnaA 669 522 chromosomal replication initiator protein dnaA dnaA_primerF CACCAGGAGCAACTTAT 49 °C
dnaA_primerR CTTCAATCGGAATGAGA
murC 592 451 UDP-N-acetyl muramate-alanine ligase murC_primerF TTTGTTTACGGTGAGGA 51 °C
murC_primerR CCACTTTGGCAGGTTTA
murE 721 592 UDP-N-acetylmuramyl tripeptide synthase murE_primerF AACACCCTCAAGACAAA 48 °C
murE_primerR ACGAATACCTTAGCACC
pepN 737 615 lysyl-aminopeptidase, aminopeptidase N pepN_primerF CGCTGAAGAGGGCGATAC 58 °C
pepN_primerR CTAACCAACGGCGGAGC
pepX 664 524 X-prolyl dipeptidyl aminopeptidase pepX_primerF ACATCCCTGTTAGTCCTG 52 °C
pepX_primerR TCTCCCTCCATCTTGTG
pyrG 736 624 CTP synthase pyrG_primerF CACTGAAGTTGGTGGGA 52 °C
pyrG_primerR CATACCGAGGCAGACAC
recA 768 617 recombinase A recA_primerF AAAGAAGGTGGCATCGC 58 °C
recA_primerR ATCGTCCTCATCTAGCTCAAC
rpoB 786 661 RNA polymerase beta subunit rpoB_primerF CATTACACGCACTACGG 47 °C
rpoB_primerR GATAACAGCATCCTCGA