Table 2.
Gene | Amplicon size (bp) | Application size (bp) | Gene function | PCR primer | Sequences (5’-3’) | Temperature |
---|---|---|---|---|---|---|
carB | 652 | 499 | carBamoyl phosphate synthetase | carB_primerF | AAGGCTACAGTGTTGTTCT | 51 °C |
carB_primerR | GATAAGGTTTGCGTTGG | |||||
clpX | 768 | 630 | ATP-dependent Clp protease subunit X | clpX_primerF | GCAGGTTATGTGGGTGA | 52 °C |
clpX_primerR | TACTGGAGCAGCTTTCC | |||||
dnaA | 669 | 522 | chromosomal replication initiator protein dnaA | dnaA_primerF | CACCAGGAGCAACTTAT | 49 °C |
dnaA_primerR | CTTCAATCGGAATGAGA | |||||
murC | 592 | 451 | UDP-N-acetyl muramate-alanine ligase | murC_primerF | TTTGTTTACGGTGAGGA | 51 °C |
murC_primerR | CCACTTTGGCAGGTTTA | |||||
murE | 721 | 592 | UDP-N-acetylmuramyl tripeptide synthase | murE_primerF | AACACCCTCAAGACAAA | 48 °C |
murE_primerR | ACGAATACCTTAGCACC | |||||
pepN | 737 | 615 | lysyl-aminopeptidase, aminopeptidase N | pepN_primerF | CGCTGAAGAGGGCGATAC | 58 °C |
pepN_primerR | CTAACCAACGGCGGAGC | |||||
pepX | 664 | 524 | X-prolyl dipeptidyl aminopeptidase | pepX_primerF | ACATCCCTGTTAGTCCTG | 52 °C |
pepX_primerR | TCTCCCTCCATCTTGTG | |||||
pyrG | 736 | 624 | CTP synthase | pyrG_primerF | CACTGAAGTTGGTGGGA | 52 °C |
pyrG_primerR | CATACCGAGGCAGACAC | |||||
recA | 768 | 617 | recombinase A | recA_primerF | AAAGAAGGTGGCATCGC | 58 °C |
recA_primerR | ATCGTCCTCATCTAGCTCAAC | |||||
rpoB | 786 | 661 | RNA polymerase beta subunit | rpoB_primerF | CATTACACGCACTACGG | 47 °C |
rpoB_primerR | GATAACAGCATCCTCGA |