Fig. 5.

DNMT1 depletion induces down-regulation of ICCG genes. The mRNA expression levels of the four representative ICCG genes and of three non-ICCG genes (KIF5B, PWP1, and CEP70) were determined by RT-qPCR in HNEM-hTERT- and BJ-hTERT-derived clones with or without exposure to doxycycline (7 days). Values, which derived from at least four independent experiments, were normalized by the ACTINB expression level and are expressed relative to the levels found in − doxy cells