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. Author manuscript; available in PMC: 2015 Oct 26.
Published in final edited form as: Curr Biol. 2013 Sep 12;23(19):1844–1852. doi: 10.1016/j.cub.2013.07.084

Figure 4. Cell Death Correlates with the Amount of Protein Aggregates.

Figure 4

(A) Bright-field (BF) and fluorescence images of a strain expressing Hsp104-GFP, and the corresponding schemes. The cell with a large amount of protein aggregates died (magenta edge), while its sister survived (white edge). The scale bar represents 1 μm. The time is given in minutes.

(B) Aggregate amount (A, Hsp104-GFP intensity in arbitrary units, a.u.) and puncta number (N) for dead cells (magenta), their sisters (green), and the population (black).

(C) Death frequency in cells born with Hsp104-GFP intensity or aggregate number above (magenta) and below (green) the death threshold, d (d=5 a.u. for A, defined as three times the average of the population; or 2 aggregates for N, see B).

The data are means ± SEM. The number of cells from more than three independent experiments is given in the graphs. See also Figures S3 and S4 and Movie S3.