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Western blotting of sCypA expression in HK-2 cells treated with different concentrations of glucose and H2O2. (A) HK-2 cells were treated with different concentrations (10, 25, and 50 mM) of glucose. All concentrations of glucose increased the expression of sCypA. (B) The expression of sCypA was increased in cells with 20 or 40 μM H2O2 treatments. They could both be counteracted by 300 U/mL catalase. Endo-CypA = endogenous cyclophilin A, sCypA = secreted cyclophilin A.
