Fig. 2.

Disruption of photoentrainment in Opn5^−/−, but not Opn3^−/−, retinas. (A) Dark-recorded bioluminescence traces (with background luminescence subtracted) from a pair of WT;Per2::Luciferase, Opn3^−/−;Per2::Luciferase or Opn5^−/−;Per2::Luciferase retinas after 4 d of culturing at the 0° (blue) or 180° (red) position of the light/dark photoentrainment apparatus. The WT data are from the same experiment as shown in Fig. 1C. (B) Time of peak bioluminescence of retinas (points, mean phase ± SEM) photoentrained at 0° (blue) and 180° (red) positions. Controls (DARK) are retinas maintained in continuous darkness. Bars indicate periods of darkness (gray) and light exposure (white; 5 W⋅m⁻²) in entrainment cycles. WT, n = 7 retinal pairs; Opn3^−/−, n = 6 retinal pairs; Opn5^−/− line 1 (main text), n = 7 retinal pairs.