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. 2015 Oct 5;112(42):13087–13092. doi: 10.1073/pnas.1514135112

Fig. 4.

Fig. 4.

Diverse cell-wall synthesis factors become required in ΔponA1 cells. (A) Transposon insertions (vertical bars), determined from high-throughput sequencing, are visualized at the ponA2 locus in either wild-type (gray) or ΔponA1 (red) cells. (B) Colony-forming units (CFUs) were counted from allelic exchanges with L5-integrating vectors that do or do not encode ponA1 in the experimental ΔponA1 L5::ponA1wt ΔponA2 strain or the control ΔponA2 strain (both control strain transformations had lawn growth, and CFUs were arbitrarily set to 6,000). (C) Transposon insertions at the rv1086 locus in wild-type (gray) and ΔponA1 (red) cells. (D) CFU counts from L5 allelic exchanges in the experimental ΔponA1 L5::ponA1wt Δrv1086 strain or the control Δrv1086 strain. (E) Transposon insertions at the ldtB locus in wild-type (gray) or ΔponA1 (red) cells. (F) CFU counts from L5 allelic exchanges in the experimental ΔponA1 L5::ponA1wt ΔldtB strain.