Fig. S1.

Schematic of genetic and chemical interaction studies in Mtb. Genome-wide genetic interactions are mapped by comparing the frequencies of transposon insertions in all loci in different genetic backgrounds using transposon mutagenesis and high-throughput sequencing. Genetic interactions can be identified by differential transposon insertion profiles in a gene in wild-type versus mutant cells. Genetic interactions can be confirmed by complementary reverse whole-genome screens or targeted knockouts. Cellular assays, for example with drug inhibition of cell-wall synthesis, can illuminate the relative contribution of genetic interactors to the targeted cell pathway. Abx^R, antibiotic resistance cassette.