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Effects of in vivo optogenetic activation of ARN kisspeptin neurons on LH secretion in GPR54-null mice. Representative examples of 5-min blue light activation of kisspeptin neurons (indicated by blue bars) at 2, 5, 10, and 20 Hz in (A) an AAV-injected Kiss1-IRES-Cre; Gpr54-null male mice and (B) in a control AAV-injected Kiss1-IRES-Cre;Gpr54+/− male mice.
