Fig. 1.

The effects of acute long-distance running in mice. All mice had access to running wheels for 3 d (A). After 2 d without wheels, half of the mice (RUN) were given again free access for 6 h, whereas controls (CON) remained sedentary. Mice were behaviorally tested subsequently, and RUN exhibited reduced anxiety-like behavior (B) and reduced thermal pain sensitivity (C). eCBs were increased after running (D). N = 16 controls and n = 16 runners for A–C, and n = 13 controls and n = 8 runners for D. *Significant difference, with P < 0.05. ***P < 0.001. Columns represent means + SE.