Coexpression network topology identifies a specific cluster associated with the transcription factor twi. The network is focused on commonly up-regulated genes (called “seeds”) in the R1, R3, R4, and R7 lines. Genes in all datasets with expression profiles highly correlated (correlation coefficient ≥0.8) to the seeds are in yellow. Seeds with similar expression profiles are found in eight clusters shown in different colors: dark or light blue, green, purple, and pink. The twi cluster is highlighted in the dashed frame. The network is shown at higher magnification in Fig. S7.