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. 2015 Oct 5;112(42):12974–12979. doi: 10.1073/pnas.1517729112

Fig. 6.

Fig. 6.

Immortalized AMP-like cells differentiate in vitro into muscle cells in response to ecdysone treatment. (A and B) Immunofluorescence staining showing Twi (anti-Twi, red) and MHC expression (anti-MHC, yellow) in cells that were mock treated (A) or treated with ecdysone (B) after 24 h. (Scale bar, 100 μm.) (C) Relative quantification of known muscle differentiation markers by qPCR, comparing ecdysone and mock-treated R1 cells. Data are shown as mean ± SE (n = 3).