Fig. S8.

Treatment of the Ras^V12 cell lines with an inhibitor of the MAPK pathway induces apoptosis. The R1, S2R⁺, and Kc cell lines were treated with either the MEK inhibitor UO126 at 10 µM or DMSO (mock treatment) after 24 h. (A) Microscope images. (B) A TUNEL assay was performed as described in SI Methods. (B, a, c, e, g, i, and k) DAPI staining is shown in blue. (B, b, d, f, h, j, and l) Apoptotic cells (red) were visualized with a fluorescent microscope. Representative samples are shown. (C and D) Controls for the effectiveness of the drug inhibiting MEK by Western blot analysis. (C) Anti-pERK and anti-ERK. (D) Ratios of pERK normalized to ERK.