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. 2015 Oct 5;112(42):12974–12979. doi: 10.1073/pnas.1517729112

Fig. S9.

Fig. S9.

CG9650 is required for proliferative activity of RasV12-expressing AMPs. (A–F) Late third-instar discs stained for Twi (anti-Twist, green) in control (A), in TubGal80ts; Dmef2-Gal4 > UAS-RasV12 (B), and in TubGal80ts; Dmef2-Gal4 > UAS-CG9650 RNAi; UAS-RasV12 (C) cells, with an optical section of the wing disc shown in D, E, and F, respectively. (Scale bars, 50 μm.) (G and H) Quantification of the number of PH3+ AMPs in the RasV12 expression using TubGal80ts; Dmef2-Gal4 > UAS-RasV12 (G) and in the simultaneous RasV12 expression and CG9650 down-regulation using TubGal80ts; Dmef2-Gal4 > UAS-CG9650 RNAi; UAS-RasV12 (H). Gal80 repression was relieved from early third instar until late third instar by shifting from 18 °C to 29 °C. All plots show mean ± SD (n = 5).