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. 2015 Oct 5;112(42):E5689–E5698. doi: 10.1073/pnas.1506129112

Fig. 3.

Fig. 3.

Replication, integration, and in vivo effects of mutant ND4. (A) Autoradiogram of agarose gel of in vitro DNA synthesis assay of transgenic liver (lane 1) and control liver mitochondria (lane 2). (B) Autoradiogram of Southern blot hybridization to labeled probe detecting human ND4. (C–F) 2D BN-PAGE reacted against NDUFS4 (S4) and NDUFA9 (A9) subunits of complex I and FLAG (C), FLAG (D), ND4 (E), and mCherry (F). (G and H) Averaged PERG waveforms with the x axis in 100-ms increments (G) and scatterplot of PERG amplitudes (H) of transgenic mice. (I and J) Scatterplots of 3-mo-old transgenic mice with low PERG amplitudes at baseline show that amplitudes increased 1 (1M) and 3 (3M) mo after intravitreal injection of mitochondria-targeted scAAV containing the wild-type human ND4 (I) but remained low in eyes injected with sterile PBS (J). (K) Bar plot of PERG amplitudes recorded 1, 3, and 6 mo after injection of rAAV-COX8Cherry. (L and M) Scatterplots of serial SD-OCT of the RGC layer and IPL of the same group of mice at 5 (5M) to 7 (7M) mo (L) and at 8 (8M) to 11 (11M) mo (M). (N) OCT images of a transgenic mouse at age 3 (3m), 7 (7m), and 15 (15m) mo.