TM dynamic material properties under normal conditions, in low pH, and with high viscosity as a function of frequency (median and interquartile range). TM shear storage modulus (A) and shear viscosity (B) under physiological conditions (blue), in reduced pH (red), and with increased viscosity (orange) are shown. Reducing bath pH reduces the TM shear storage modulus, with little change in shear viscosity. Conversely, increasing bath viscosity with PEG primarily increases TM shear viscosity, with little change in the shear storage modulus.