Fig. 4.

Kdelr1 mutation is causative for daniel gray phenotype. (A) Schematic of the Cas9/sgRNA targeting site at the Kdelr1 locus. Boxes indicate exons, with dark shading indicating protein-coding sequence. The sgRNA targeting sequence is underlined, and the protospacer-adjacent motif (PAM) is labeled in red. (B) Frequencies and CD44 mean fluorescence intensity (MFI) of CD4⁺ and CD8⁺ blood lymphocytes, including those from a CRISPR/Cas9 engineered mutant of Kdelr1 (U2077). CRISPR WT mice represent nonmutant littermates of U2077. (C) Frequencies and CD44 MFI of CD4⁺ and CD8⁺ blood lymphocytes, including those from daniel gray mice with a wild-type Kdelr1 transgene [Tg(Kdelr1)]. P values were calculated by unpaired t test. Each symbol in B and C represents an individual mouse (error bars represent SEM).