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Immunochemistry to define apoptosis by enumerating cells expressing cleaved caspase-3. (A) The absolute number of cleaved caspase-3+ cells for seven patients before and 4–10 d after treatment with 90Y-daclizumab. The absolute number of cleaved caspase-3+ cells was counted at 40× magnification over five high-power fields focusing on Reed–Sternberg cells. (B) Representative immunochemistry fields for patients A and F before treatment (Left) and 4–10 d after treatment (Right).
