Skip to main content
. 2015 Oct 5;112(42):E5669–E5678. doi: 10.1073/pnas.1516219112

Fig. 4.

Fig. 4.

PGC-1α and HSF1 interact physically and coreside on target gene promoters. (A) Coimmunoprecipitation of PGC-1α and HSF1 in HEK293 nuclear extracts. Nuclear extracts from HEK293 cells expressing V5-tagged HSF1 and FLAG-PGC-1α or control vector were subjected to immunoprecipitations using an anti-FLAG antibody. Immunoprecipitates (IP) and nuclear extracts (NE) were blotted and probed with the indicated antibodies. (B) Recombinant bacterially expressed purified proteins used in C. Purified proteins were subjected to SDS/PAGE and stained with Coomassie Blue. (C) PGC-1α and HSF1 interact directly in vitro. Purified PGC-1α was incubated with immobilized GST or GST-HSF1. After washing the retained material was resolved by SDS/PAGE, blotted, and probed as indicated. The lower band is a likely degradation product of FLAG-PGC-1α. (D) ChIP analysis for HSF1 in HepG2 cells. Values represent enrichment of selected target gene promoters over an upstream region of the GAPDH promoter. (E) ChIP analysis for PGC-1α in HepG2 cells. Values represent the enrichment of selected target gene promoters over an upstream region of the GAPDH promoter.