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. 2015 Oct 5;112(42):E5669–E5678. doi: 10.1073/pnas.1516219112

Fig. 7.

Fig. 7.

PGC-1α regulates non-HSR HSF1-dependent transcriptional programs. (A) RT-PCR validation of mRNA expression of several non-HSR genes identified as repressed by PGC-1α in HepG2 cells in our microarray analysis. (B) Non–HSR-related terms found in term enrichment analysis of genes repressed 1.5-fold or more by PGC-1α expression in HepG2 cells. (C) Heat map representing fold repression by PGC-1α of selected genes in cells transfected with control siRNAs (SiControl) or with siRNAs targeting HSF1 (SIHSF1). (D) RT-PCR analysis of target gene mRNA fold repression by PGC-1α in HepG2 cells that were transfected with siRNAs targeting HSF1 (SiHSF1) or with control siRNAs (SiControl). (E) RT-PCR analysis of mRNA expression of several non-HSR genes repressed by PGC-1α in HeLa cells. (F) ChIP analysis for HSF1 in HepG2 cells. Values represent enrichment of selected target gene promoters over an upstream region of the GAPDH promoter. (G) ChIP analysis for PGC-1α in HepG2 cells. Values represent enrichment of selected target gene promoters over an upstream region of the GAPDH promoter.