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Detection of wild poliovirus type 1 (WPV1) by reverse transcription-semi-nested polymerase chain reaction in stool incubated at to elevated temperatures over time. (A). WPV1-containing stool exposed for 4–28 days at 35 °C or 45 °C, and for 28 days at 25 °C. (B). WPV1-containing stool exposed for 1–14 days at 31 °C or 35 °C, and for 14 days at 27 °C.
