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CD45+ hematopoietic cells (panel A), CD45− nonhematopoietic cells (panel B) and lymphoid tissue inducer cells [lineage-CD3−CD4+CXCR5+(LTi)] (panel C) from the mediastinal lymph nodes (MLN) of sham-irradiated and irradiated neonate mice were counted by flow cytometry at 7 days post irradiation. Total numbers of CD19+ B cells (panel D), CD3+CD4+ T cells (panel E) and CD11c+ dendritic cells (panel F) are also shown. n = 5 mice/group. Graphs represent mean ± SEM. *P < 0.05; ****P < 0.0001.
