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. 2015 Oct 20;6:858. doi: 10.3389/fpls.2015.00858

FIGURE 4.

FIGURE 4

Donor species genes can be screened for phenotypic effects in the recipient species by shotgun or clone-by-clone strategies. In a shotgun screen (left half of flow-chart) the library is transferred en masse into recipient plants, which are screened phenotypically. In a clone-by-clone screen (right half of flow-chart), clones are arranged in microtiter plates and transformants are generated for each isolated clone.