Table 1.
List of commercially available antibodies used in analyses of protein-DNA interactions in filamentous fungi.
| Company | Epitope | Catalog # | type | beads | ChIP | Western |
|---|---|---|---|---|---|---|
| abcam | GFP | ab290 | rabbit polyclonal | protein A | excellent | yes |
| Active Motif | H3K9acetyl | 39137 | rabbit polyclonal | protein A | yes | yes |
| Active Motif | H3K14acetyl | 39127 | rabbit polyclonal | protein A | yes | yes |
| Active Motif | H3K27acetyl | 39133 | rabbit polyclonal | protein A | yes | yes |
| Active Motif | H3 C-terminus | 39163 | rabbit polyclonal | protein A | no | yes |
| Active Motif | H3S10,28phospho | 39147 | rabbit polyclonal | protein A | no | yes |
| Active Motif | H3K4trimethyl | 39159 | rabbit polyclonal | protein A | excellent | yes |
| Active Motif | H3K9dimethyl | 39240 | rabbit polyclonal | protein A | yes | yes |
| Active Motif | H3K9trimethyl | 39161 | rabbit polyclonal | protein A | excellent | yes |
| Active Motif | H3K27trimethyl | 39535 | mouse-monoclonal | protein G | excellent | yes |
| Active Motif | H4K16acetyl | 39167 | rabbit polyclonal | protein A | yes | yes |
| Active Motif | H4K20trimethyl | 39181 | rabbit polyclonal | protein A | yes | yes |
| Sigma | FLAG | F3165 | mouse-monoclonal | protein G | ok | yes |
| Roche | HA | 11 867 423 001 | rat monoclonal | protein G | no | yes |
| abcam | H3K9acetyl | ab4441 | rabbit polyclonal | protein A | yes | yes |
| Millipore | H3K4dimethyl | 07–030 | rabbit polyclonal | protein A | excellent | yes |
| Active Motif | H3K27trimethyl | 39155 | rabbit polyclonal | protein A | yes | yes |
| Active Motif | H3K9acetyl | 39137 | rabbit polyclonal | protein A | yes | yes |
| Millipore | H3K14acetyl | 07–353 | rabbit polyclonal | protein A | yes | yes |
| Active Motif | H3K27ac | 39133 | rabbit polyclonal | protein A | yes | yes |
| Millipore | H3K27me2 | 07–452 | rabbit polyclonal | protein A | yes | yes |
| Abcam | H3K9trimethyl | ab8898 | rabbit polyclonal | protein A | ok | no |
| Millipore | H3S10ph | 04–817 | rabbit polyclonal | protein A | no | yes |
| Millipore | H4 | 05–858 | rabbit polyclonal | protein A | no | yes |
| Millipore | H3 | 07–690 | rabbit polyclonal | protein A | no | yes |
| abcam | H3K36me3 | ab9050 | rabbit polyclonal | protein A | yes | yes |
| Active Motif | RNA PolII CTD S5ph | 39233 | rabbit polyclonal | protein A | yes | yes |
| abcam | V5 | ab9116 | rabbit polyclonal | protein A | yes | yes |
| Invitrogen | V5 | R960–25 | rabbit polyclonal | protein A | excellent | yes |