Figure 4. Vaccination with GPI-HER-2-VLPs enhances Th1-type antibody responses.
Mice were vaccinated with GPI-HER-2-VLPs (25 μg) on day 0 and day 14, and serum was collected on day 21. Anti-D2F2/E2-specific serum (A) IgG1, (B) IgG2a, and (C) IgG2b subtype antibodies were analyzed by cell ELISA using 1:200 diluted serum from vaccinated mice (n = 5) with 50,000 D2F2/E2 cells per well.