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. Author manuscript; available in PMC: 2015 Oct 26.
Published in final edited form as: J Asthma. 2013 Jan 9;50(2):122–132. doi: 10.3109/02770903.2012.753455

Figure 3.

Figure 3

Quantification of lung tissue CD4 + Foxp3+ cells. Twenty-four hours after the final antigen challenge, lung lymphocytes were isolated and stained with Pacific Blue-conjugated Live/Dead, and live cells were gated on CD45 followed by CD4 and Foxp3. Regulatory T-cells were expressed as a percentage of total CD4+ T-cells in the lung digests. (A) Representative gating strategy from an 18-month non-fed/OVA mouse. (B) The proportion of CD4+ T-cells that are Foxp3 +. Data are expressed as means ± SEM from combined data from three independent experiments. (Total numbers of mice: n = 13/group, 6-week non-fed/OVA, 6-week OVA-fed/ OVA; n = 8/group, 18-month OVA-fed/OVA, 18-month non-fed/OVA; n = 7, 6-week naïve; and n = 6, 18-month naïve mice). *p < .05, **p < .01.