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. Author manuscript; available in PMC: 2015 Oct 26.
Published in final edited form as: J Asthma. 2013 Jan 9;50(2):122–132. doi: 10.3109/02770903.2012.753455

Figure 5.

Figure 5

Effect of age and oral OVA administration on lung tissue inflammation and goblet cell metaplasia. Forty-eight hours after the final OVA challenge, lung tissue was surgically removed and fixed. (A, 1–4) Representative PAS stained lung sections from mice demonstrating reduced mucus (deep purple) with OVA feeding. (A, 5–8) Representative H&E stained lung sections demonstrating reduced cellular infiltration surrounding bronchi (PB) and vessels (PV) with OVA feeding in both aged and younger mice. H&E stained tissue was graded for both (B) perivascular (PV) and (C) peribronchial (PB) inflammation, as described in Methods. (D) Bronchiolar mucus metaplasia was accessed by PAS tissue staining, as described in Methods. (Total numbers of mice: n=10, 6-week non-fed/OVA, 6-week OVA-fed/OVA, 18-month non-fed/OVA; n = 14, 18-month non-fed/OVA; n = 7, 6-week naïve; and n = 5, 18-month naïve mice). *p < .05, **p < .01, ***p < .001. Scale bars: PAS-200 µm, H&E-500 µm.